Hypothyroidism induced by propylthiouracil decrease sirtuin1 content in rat heart

Qi-Yao Xu, Xiao-Long Wang, You-Fan Peng


Background: In this study, the changes in sirtuin1 (SIRT1) levels in the heart of rats with hypothyroidism (HT) were evaluated.
Methods: A total of 20 Wistar male rats were randomly divided into control groups (n=10) and HT groups (n=10). In the HT groups, we used 0.05% propylthiouracil (PTU) saline solution to prepare the HT model, while 0.9% sodium chloride solution was used as a placebo in the control groups. During the intervention period, the body weight of rats was measured weekly, and serum triiodothyronine (TT3), tetraiodothyronine (TT4), thyroid-stimulating hormone (TSH), body weight, heart weight, and SIRT1 content in the heart were measured after intervention. We compared the differences in SIRT1 content between the two groups and analyzed the correlation between SIRT1 and thyroid hormone concentrations.
Results: After 8 weeks of intervention, the HT rat model was successfully produced. The body weight of the HT group rats increased more slowly than that of the control groups. The heart weight of the HT groups was significantly lower than that of the control groups, and SIRT1 content decreased significantly compared to in control group rats. However, there were no significant differences between the two groups in heart weight index. The relative content of SIRT1 was significantly correlated with TT3 concentrations, both in the control group and HT group.
Conclusions: Our results suggest that HT induced by PTU significantly reduces myocardial SIRT1 content. Decreased TT3 was significantly associated with decreased SIRT1 content.