%0 Journal Article %T Pseudothrombocytopenia induced by incubation at 37 ℃ in ethylenediaminetetraacetic acid tubes %A Zhang, Lixia %A Wang, Lin %A Gao, Li %A Xu, Jian %A Pan, Shiyang %J Journal of Laboratory and Precision Medicine %D 2017 %B 2017 %9 %! Pseudothrombocytopenia induced by incubation at 37 ℃ in ethylenediaminetetraacetic acid tubes %K %X Ethylenediaminetetraacetic acid (EDTA) is the anticoagulant currently used for cell blood counts. Several studies showed that exposure of platelets (PLTs) to EDTA for a short period of time promote tyrosine phosphorylation of certain proteins, events that are related to activation. Spuriously low PLT counts were observed in a patient with cold agglutinins after the blood being warmed at 37 ℃ in EDTA tubes. Some cloudy polymers were found on peripheral blood smear after incubation at 37 ℃ for 15 minutes. These data suggest that PLT were activated after being warmed to 37 ℃ with EDTA. This process initiated coagulation and generated fibrin and PLT polymers from PLT agglutination and release. This case report hence describes a very rare phenomenon, which produces a spuriously low PLT counts caused by warming of EDTA tubes at 37 ℃. %U https://jlpm.amegroups.org/article/view/3594 %V 2 %P %@ 2519-9005