Guest editor
Dirk Roggenbuck, PhD
GA Generic Assays GmbH, L.-Erhard-Ring 3, Berlin, Germany; Institute of Biotechnology, Faculty of Environment and Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany
DNA damage analysis is an evolving diagnostic field, particularly for precision medicine, that opens new avenues for the use of potential biomarkers such as the phosphorylated histone 2Ax (γH2AX), 8-hydroxy-2'-deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). In this focused issue, Reddig et al. highlight the role of γH2AX as a promising marker for DNA double strand breaks (DSB) in the context of precision medicine in a comprehensive review. Reddig et al. provide an overview of the applicability of genotoxicity methods in that context and underline the benefits of fluorescence microscopy with its superior sensitivity for γH2AX foci counting in the case of DSB assessment in a comparative analysis with other γH2AX-detecting methods and etc.
Preface
DNA damage assessment for precision medicine: an emerging diagnostic option
Dirk Roggenbuck
Original Article
Middle-distance running and DNA damage in diabetics
Giuseppe Lippi, Cantor Tarperi, Elisa Danese, Martina Montagnana, Luca Festa, Marco Benati, Gian Luca Salvagno, Chiara Bonaguri, Elisabetta Bacchi, Silvia Donà, Dirk Roggenbuck, Paolo Moghetti, Federico Schena
Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells
Mandy Sowa, Annika Reddig, Peter Schierack, Dirk Reinhold, Dirk Roggenbuck
Quantification of DNA double-strand breaks in peripheral blood mononuclear cells from healthy donors exposed to bendamustine by an automated γH2AX assay—an exploratory study
Stefan Rödiger, Marius Liefold, Madeleine Ruhe, Mark Reinwald, Eberhard Beck, P. Markus Deckert
Comparison of different immunoassays for γH2AX quantification
Annika Reddig, Dirk Roggenbuck, Dirk Reinhold
Review Article
DNA damage assessment and potential applications in laboratory diagnostics and precision medicine
Annika Reddig, Claudia E. Rübe, Stefan Rödiger, Peter Schierack, Dirk Reinhold, Dirk Roggenbuck
Detection of 8-OHdG as a diagnostic biomarker
Kemal Sami Korkmaz, Bilge Debelec Butuner, Dirk Roggenbuck
Molecular biomarkers of DNA damage in diffuse large-cell lymphoma—a review
Kemal Sami Korkmaz, Bilge Debelec Butuner, Dirk Roggenbuck
Open source bioimage informatics tools for the analysis of DNA damage and associated biomarkers
Jens Schneider, Romano Weiss, Madeleine Ruhe, Tobias Jung, Dirk Roggenbuck, Ralf Stohwasser, Peter Schierack, Stefan Rödiger
Disclosure:
The series “DNA Damage Assessment for Precision Medicine” was commissioned by the editorial office, Journal of Laboratory and Precision Medicine without any sponsorship or funding. Dirk Roggenbuck served as the unpaid Guest Editor for the series.
