DNA damage assessment for precision medicine

Posted On 2019-06-06 17:45:39

Guest editor
Dirk Roggenbuck, PhD
GA Generic Assays GmbH, L.-Erhard-Ring 3, Berlin, Germany; Institute of Biotechnology, Faculty of Environment and Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany

DNA damage analysis is an evolving diagnostic field, particularly for precision medicine, that opens new avenues for the use of potential biomarkers such as the phosphorylated histone 2Ax (γH2AX), 8-hydroxy-2'-deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). In this focused issue, Reddig et al. highlight the role of γH2AX as a promising marker for DNA double strand breaks (DSB) in the context of precision medicine in a comprehensive review. Reddig et al. provide an overview of the applicability of genotoxicity methods in that context and underline the benefits of fluorescence microscopy with its superior sensitivity for γH2AX foci counting in the case of DSB assessment in a comparative analysis with other γH2AX-detecting methods and etc.

Preface

DNA damage assessment for precision medicine: an emerging diagnostic option

Dirk Roggenbuck

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Original Article

Middle-distance running and DNA damage in diabetics

Giuseppe Lippi, Cantor Tarperi, Elisa Danese, Martina Montagnana, Luca Festa, Marco Benati, Gian Luca Salvagno, Chiara Bonaguri, Elisabetta Bacchi, Silvia Donà, Dirk Roggenbuck, Paolo Moghetti, Federico Schena

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Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells

Mandy Sowa, Annika Reddig, Peter Schierack, Dirk Reinhold, Dirk Roggenbuck

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Quantification of DNA double-strand breaks in peripheral blood mononuclear cells from healthy donors exposed to bendamustine by an automated γH2AX assay—an exploratory study

Stefan Rödiger, Marius Liefold, Madeleine Ruhe, Mark Reinwald, Eberhard Beck, P. Markus Deckert

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Comparison of different immunoassays for γH2AX quantification

Annika Reddig, Dirk Roggenbuck, Dirk Reinhold

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Review Article

DNA damage assessment and potential applications in laboratory diagnostics and precision medicine

Annika Reddig, Claudia E. Rübe, Stefan Rödiger, Peter Schierack, Dirk Reinhold, Dirk Roggenbuck

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Detection of 8-OHdG as a diagnostic biomarker

Kemal Sami Korkmaz, Bilge Debelec Butuner, Dirk Roggenbuck

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Molecular biomarkers of DNA damage in diffuse large-cell lymphoma—a review

Kemal Sami Korkmaz, Bilge Debelec Butuner, Dirk Roggenbuck

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Open source bioimage informatics tools for the analysis of DNA damage and associated biomarkers

Jens Schneider, Romano Weiss, Madeleine Ruhe, Tobias Jung, Dirk Roggenbuck, Ralf Stohwasser, Peter Schierack, Stefan Rödiger

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